Morphological, biochemical and molecular characterization of Egyptian clover (Trifolium alexandrinum L.) varieties

Authors

  • M. M Tarrad Forage Crops Research Department, Field Crops Research Institute, Agricultural Research Center, Giza 12619, Egypt
  • E. M. Zayed Forage Crops Research Department, Field Crops Research Institute, Agricultural Research Center, Giza 12619, Egypt

Keywords:

Egyptian clover, Field traits, Isozymes, PAGE, RAPD

Abstract

Seeds of five Egyptian clover varieties, Helaly, Serw 1, Gemmiza 1, Giza 6 and Sakha 4 were planted during 2007 at Giza Agricultural Research Farm, Egypt. The highest total dry matter yield of 15.86 t ha-1 was recorded for Gemmiza 1 variety. Polyacrylamide gel electrophoresis (PAGE) was performed for native and SDS protein and isozyme variations. RAPD was conducted using 8 arbitrary 10-mer primers. Combined analysis based on four isozymes, PAGE protein electrophoresis and RAPD analyses revealed highest similarity of 0.85 between the two varieties Sakha 4 and Gemmiza 1, while the lowest similarity (0.53) was observed between Giza 6 and Helaly.

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01-12-2009
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How to Cite

M. M Tarrad, & E. M. Zayed. (2009). Morphological, biochemical and molecular characterization of Egyptian clover (Trifolium alexandrinum L.) varieties. Range Management and Agroforestry, 30(2), 115–121. Retrieved from https://publications.rmsi.in/index.php/rma/article/view/25

Issue

Vol. 30 No. 2 (2009)

Section

Articles